Cleanroom Specifications
U.S. Federal Standard 209E Cleanroom Standards*
| Class | Maximum Particles / ft³ | ISO Equivalent |
||||
| ≥0.1 µm | ≥0.2 µm | ≥0.3 µm | ≥0.5 µm | ≥5 µm | ||
| 1 | 35 | 7 | 3 | 1 | ISO 3 | |
| 10 | 350 | 75 | 30 | 10 | ISO 4 | |
| 100 | 750 | 300 | 100 | ISO 5 | ||
| 1,000 | 1,000 | 7 | ISO 6 | |||
| 10,000 | 10,000 | 70 | ISO 7 | |||
| 100,000 | 100,000 | 700 | ISO 8 | |||
*US FED STD 209E was cancelled by the General Services Administration on November 29, 2001.
IEST ISO 14644-1 International Standard for Cleanrooms more information
| Class | Maximum Particles / m³ | U.S. Federal Standard 209E Equivalent |
|||||
| ≥0.1 µm | ≥0.2 µm | ≥0.3 µm | ≥0.5 µm | ≥1 µm | ≥5 µm | ||
| ISO 1 | 10 | 2 | |||||
| ISO 2 | 100 | 24 | 10 | 4 | |||
| ISO 3 | 1,000 | 237 | 102 | 35 | 8 | Class 1 | |
| ISO 4 | 10,000 | 2,370 | 1,020 | 352 | 83 | Class 10 | |
| ISO 5 | 100,000 | 23,700 | 10,200 | 3,520 | 832 | 29 | Class 100 |
| ISO 6 | 1,000,000 | 237,000 | 102,000 | 35,200 | 8,320 | 293 | Class 1000 |
| ISO 7 | 352,000 | 83,200 | 2,930 | Class 10,000 | |||
| ISO 8 | 3,520,000 | 832,000 | 29,300 | Class 100,000 | |||
Biosafety Levels
Biosafety levels define the levels of containment required for handling various types of biological hazards. In the United States, there are four levels of containment as specified by the Centers for Disease Control and Prevention (CDC). The table below is based on information provided by in CDC publications.| Containment Level | Description |
| Biosafety Level 1 (BSL-1) | Basic level of protection, appropriate for agents that are not known to cause disease in normal, healthy humans. |
| Biosafety Level 2 (BSL-2) | Level of protection appropriate for handling moderate-risk agents that cause human disease of varying severity by ingestion or through percutaneous or mucous membrane exposure. |
| Biosafety Level 3 (BSL-3) | Level of protection appropriate for agents of indigenous of exotic origin with a know potential for aerosol transmission that may cause serious and potentially lethal infections after inhalation. |
| Biosafety Level 4 (BSL-4) | Highest level of protection, appropriate for exotic agents that pose a high individual risk of life-threatening disease by infectious aerosols and for which no treatment is available. |
Related Links
Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition Published by the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention
and National Institutes of Health; Fifth Edition, Feb 2007
European Standard
| Grade | Air Changes per Hour |
| Grade A | 480-600 |
| Grade B | 35-70 |
| Grade C | 20 |
| Grade D | 20 |